Hi all,
Sorry of this is a basic question to ask but I haven' t much experience with this side of things and any and all help would be greatly appreciated.
I am using GAPDH as my normalizing protein but am unsure of how to go about it. At present I am using Licor software, I am drawing a box around my GAPDH band and getting a value "X". For the other proteins I am analyzing I am repeating this and getting a value "Y"
Do I divide Y by X? And what do I do after?
Thanks for your help