Hi all,

Sorry of this is a basic question to ask but  I haven' t much experience with this side of things and any and all help would be greatly appreciated. 

I am using GAPDH as my normalizing protein but am unsure of how to go about it. At present I am using Licor software,  I am drawing a box around my GAPDH band and getting a value "X". For the other proteins I am analyzing I am repeating this and getting a value "Y" 

Do I divide Y  by X? And what do I do after? 

Thanks for your help

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