Recently, I was able to extract and precipitate thrombin (an enzyme used in Hemostasis).
Extraction and precipitation were carried out using ETHANOL.
Now, I need to purify it (purified this precipitin thrombin which contains many other molecules other than thrombin)
I know that chromatography is the best way to purify this thrombin. But what type of chromatography (Sephadex, Saphros Cellulose and what kind of buffer must I use)
Is there any other means other than chromatography for this kind of purification?
Thank you very much in advance for your help
Hi you may read these two papers about it, I hope it will be useful for your purpose.
Kind regards.
Rapid sulfopropyl disk chromatographic purification of bovine and human thrombin. Analytical Biochem. 157:1,15 Aug 1986 77-81.
A rapid simplyfied purification of bovine thrombin Ding and Zu
Preparative Biochem 1995 25;1-2: 21-28
Thank you very much Nilo Bentancor
These two articles seems to be very intresting unfortunatly they are not Open Acces
Best regards
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