my protein is 151 kDa, but its Instability index over 80, so it may be unstable in buffer.
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
02 December 2020 9,413 3 View
Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...
01 December 2020 1,338 5 View
I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...
21 November 2020 5,467 2 View
As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.
05 November 2020 5,946 4 View
Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.
19 October 2020 5,160 3 View
I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...
12 October 2020 4,929 3 View
how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?
23 September 2020 7,692 2 View
Hi anyone experienced in studying antibody-antigen binding with biacore (SPR) know should 1:1 binding model be used in kinetics and affinity analysis? Or should we use 1:2(antibody:antigen)? Thanks!
26 August 2020 4,130 3 View
The anthropogenic pratices are getting common these days and many catchments experience water managements e.g., drainage, which has huge impacts on both discharge and water quality dynamics. So...
13 August 2020 1,801 11 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?
02 March 2021 4,565 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
It should be standard journal (Indexed)
01 March 2021 8,379 1 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Dear Researchers, I would like to find some (Q2) Scopus-Indexed journals with fast publication in the field of Computer Science and Engineering major?
28 February 2021 3,285 2 View
please provide me the most recent Scopus Index Journals List, February-2021
27 February 2021 3,545 1 View
What are the criteria to be followed to develop the researcher's index?
26 February 2021 9,051 0 View