I would like to know the procedure that use for purification of siderophore from Bacillus megaterium culture. I have tried to use nickel column to purify but it's not work, no siderophore in eluted fraction when measure by TLC assay.
If anyone have some suggestion please help me. Thank you very much
Here is a thesis about hydroxamate siderophore purification.
http://dc.etsu.edu/cgi/viewcontent.cgi?article=2207&context=etd
@Omar Gonzalez-Ortega
Do you know how to prepare Ferric column or Is it has a commercial ferric column?
You can use the column you have. Strip the nickel ions and load ferric ions.
You can use the column you have. Strip the nickel ions and load ferric ions.
You can use the column you have. Strip the nickel ions and load ferric ions.
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