As per the protocols we use no. of chemicals for purification so is there any novel chemical which results in minimal use of other chemicals for enzyme purification? or combination of physical and chemical methods will be beneficial for purification?
Dear Nilish, the characterization is different from the downstream process. so you must have to specify your question for appropriate answer
I hope this would help. Some enzyme separation processes are related to size based separation. The one commonly used is ultrafiltration. Ultrafiltration membranes with MW-cutoffs ranging from 5000-100,000 Daltons are useful for concentrating enzymes (proteins). The protein to be filtered is placed on certain chamber and centrifugal force or vacuum is used to create the filtrate which will be accumulating in the other chamber.
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