How to purify different fractions from minute samples?

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M. Rajamohamed Kalanjiam

Dear Bhusan,

I suggest you to try with the micro scale preparative RP HPLC system set up to purify proteins from sample sizes ranging from 100 µL to 50 mL.

Niladri Bhusan Kar

Hi MRK

problem is we only have a waters analytical HPLC...can you suggest anything else?? Also the injector loop volume is 10ul...can i use what you have suggested? if so please let me know which column to use?

thanks for the answer...