Niladri Bhusan Kar

4 Questions 27 Answers 0 Followers

Questions related from Niladri Bhusan Kar

How can I make the newly synthesized cDNA strands sticky?

I have isolated the mRNA and already made the cDNA now I face the problem of making the cDNA end sticky. Can anyone suggest me how to make the ends sticky for inserting into pmal plasmid?

01 January 2014 487 4 View

How to start characterizing proteins and purify from unknown samples?

I have venom samples from organisms (species) which has never been worked with. Would anybody help me to understand how to start to characterize the different proteins?

07 July 2013 3,919 13 View

How to purify different fractions from minute samples?

I am working with proteinaceous secretion of some animals. I get them in very small quantity, around 100ul max. I want to purify the sample into different fractions and would like to perform some...

06 June 2013 5,143 2 View

How to purify protein when the sample is very Minute in Quantity (around 100ul)?

Well the sample that we have in hand is very less and cannot be procured in very high quantity. Can anybody suggest a method to segregate it for further bioassays? My samples are proteinaceous...

06 June 2013 9,151 6 View