knockdown of one gene A in undifferentiated cells cause reduction of metabolism rate and cell differentiation, while differentiated cells also show lower metabolism rate compare to undifferentiated cells.
how to prove whether the reduction of metabolism rate is a direct effect of knockdown gene A, or it is an indirect effect of the cell differentiation triggered by the loss of gene A?
Adds on: knockdown of gene A in differentiated cells (MEFs) did not change the metabolism rate.
Adam B Shapiro
You need to find a way to separate the two effects. Is there a mutant that does not respond to gene A knockdown by differentiating? If so, you could use that mutant to test whether gene A knockdown causes reduction of metabolism in the absence of differentiation.
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