Sue yu

5 Questions 4 Answers 0 Followers

Questions related from Sue yu

Anyone knows ideal neomycin selection concentration in mouse ES cells (E14)?

I will transfect a plasmid contains neomycin selection marker into mouse ES cells (E14 cell line)? This is my first time to use neomycin selection marker, so I donot know which concentration of...

12 December 2017 2,317 0 View

How to prove the reduction of metabolism rate is direct or indirect effect cause by knockdown of one gene A?

knockdown of one gene A in undifferentiated cells cause reduction of metabolism rate and cell differentiation, while differentiated cells also show lower metabolism rate compare...

09 September 2016 952 1 View

Which is better to detect protein-RNA binding, RNA-IP or RNA-CLIP?

The protein X I am studying may related to RNA binding, so I am interested to test the RNA that can be bind by protein X. I read some paper, and found that there are several kind of methods and...

04 April 2016 252 4 View

How it is possible that someone did real time PCR to check intron expression level with cDNA as template?

In some papers, I found they did real time PCR to check introns or (exon - intron) expression level for some genes using cDNA as template. The cDNA is generated by RNA extraction and followed by...

11 November 2015 1,307 7 View

How and where I check one protein is conserved in mouse and human?

Anyone knows the answers for this question, many thanks.

07 July 2015 1,267 8 View