How to properly include an IgG isotype control for flow analysis?

31 January 2020 1 9K Report

Hi,

So my problem is that I have been trying to include an IgG isotype control in my flow analysis but I get background. I'm staining bulk mouse splenocytes, I incubate with FC block and my FACS buffer contains FCS. Even at a 1:500 dilution of the IgG isotype-APC antibody, I have very clear signal. I have tried everything and nothing seems to work. Please, any advice?

Laura

Qasim Jawad Al-Daami

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