You can go for Fatty acid methyl ester (FAME) synthesis directly from the sample by following method:
O’Fallon JV, Busboom JR, Nelson ML and Gaskins CT (2007). A direct method for fatty acid methyl ester (FAME) synthesis: Application to wet meat tissues, oils and feed stuffs. J Anim Sci 85(6):1511-1521.
Then you can use the FAME present in Hexane for fatty acid profile analysis using GLC.