Dear all,
Hi,
As it is shown in the attached figure, we have dsRNA on a PAGE. However, the wavy bands and skewing on the left-side of the columns have made some disturbances. How can we prevent such problems during electrophoresis?
Regards
Dear Alireza,
It generally happens when the bottom of the well is not even. The bottom surface of the well became rough either by the comb or by the needle of sample loading syringe.
Ensure that your well surface is regular and even. A good care and attention is also a must during sample loading in the well, the tip of the needle should not reach to the bottom of the well.
regards,
Dr A K Dubey
This is a good gel so not much needs changing. Wash the wells before loading and clean the comb and possibly give the gel a little more setting time but I think the problem is too much salt in the sample leading to irregular entry of the rna into the gel. The strongest banded samples seem most effected so maybe some salt is carrying over from the purification process. Possibly run less of these samples or get rid of salts
Hi there,
To ensure a perfectly regular well bottom, make sure it doesn't make any contact at any point with air (ie. remove the comb once the gel is in the running buffer) and then flush away the polyacrylamide debris within the well with a large volume of buffer. But as Paul mentioned the pattern is not that bad...
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