How to prevent protein translation initiation from a specific ATG codon?

03 August 2017 1 239 Report

Dear all,

I would like to express a specific protein isoform from a cDNA. This protein is translated from the same mRNA as a smaller isoform whose initiation starts 80 AA after the one I want to express. Would you have a suggestion to only express the big isoform without mutating the second ATG initiation codon?

Thank you in advance for your answers.

Noor Idan Jarad

https://en.wikipedia.org/wiki/Start_codon

https://www.ncbi.nlm.nih.gov › NCBI › Literature

https://biology.stackexchange.com/

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