I am using a transgenic mouse which is an Insulin-rttaXTet-DTA with the aim of killing beta cells. In spite of giving the mice doxycycline, the insulin cells are still present. Any suggestions on how I can check if the Tet-Dta system is working?
If its a Tet-DTA system, then wouldnt it be the absence of tetracycline (doxycycline) that induces DTA expression? http://www.jneurosci.org/content/27/44/11925.full.pdf