I had to perform a MIC test on Scedosporium apiospermum last week (isolated from a BAL). Unfortunatly I couldn't use the specific and required media I was suppose to (potato's agar to select the conidia and R.P.M.I. for the E-test). I was in a sort of rush and then I decided to perform the E-test on Sabourad agar. I know that it's not correct, but I need to know if the results I obtained are reliable or not. Can I use them as an indication for the therapy?
I tested Voriconazole (which looks Sensible) Itraconazole (which has a strange MIC of 0.5 but I think is dose-related) Ketoconazole (sensible as VOR) and Caspofungin (that's surely resistant).
Any suggestion? Can I confirm to the clinical doctor this results?
(He already started a VOR therapy, and the patient seems to feel, after few days, better. But the doctor wants a confirm anyway).
Thank you, but my problem indeed, is that on SDA Scedosporium doesn't produce enough conidia to be sure that the test is reliable. I usually, as protocol, isolate it on PDA and use a sospension taken from it to perform the E-test on R.P.M.I., I would never perform it directly on PDA.
The doubt I had was about the result had on SDA, because usually I use R.P.M.I. to perform E-test.
Is a result obtained on SDA comparable with the one I would have obtained on R.P.M.I.?
Thank you.
To the best of my knowledge, the results of fungal E-test on SDA are not correct, because this medium contains some proteins. This information was obtained by me on Veterinary Mycology Courses at Radboud University (Holland).
Best regards
Thank you dr. Ovchinnikov , I heard something like that, that's why I had this doubts. I have to find a way to perform E-test without RPMI that I cannot have.
Thank you again.
You are wellcome, Ramona. Good luck in your work, and please notice us, if you will find the solution.
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