I tried to isolate RNA from çatharanthus plant which contains more phenolic components. I couldn't isolation RNA. I used DEPC treated tips and all utilities and used Quigen kit, Trizol and isoplus method and CTAB methods. Can you suggest me any solutions preparation methods for preventing RNase from sample and lab utalites. I saw using chloroform which degragiding RNAse.
Hi Ganesan Mahendran What part of the plant did you isolate the RNA from?
How many (mg) of plant tissue did you use?
How did you degrade/homogenize the tissue?
How long did you do it?
For this type of leaves I use column based (guanidin thiocyanathe) kits. I have never work exactly with this species, but it seems that leaves not so abundant in carbohydrates.
it seems that RNAses is not 100% the reason of your troubles.
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