We would put agar plugs in 25% glycerol and stick them in the minus 80. I will say that 25% is not appropriate for all species. I would test a range between 10% and 25% to see what gives the best recovery rates.

Dear William J Davis thank you so much for your response!

Sterile tap water.

Hector Urbina Thanks for your response! Do you put part of the colony in sterile water and freeze it?

Also can put agar plugs in sterile mineral oil. Store at room temperature. It is useful for many species and fungi remain well preserved for couple of years.

Keilor Rojas-Jimenez thank you very much for your good suggestion.

The fungal strains can be maintained in 20% glycerol at –80 °C, sterile mineral oil, or even in slant using a conical tube with PDA.

Thank you Kallol Das for your help!

Dear Nasim Maghboli,

You can prepare 20% glycerol solution and can preserve your fungal sample in it at -80 °C it works as we did the same in our lab

Thanks

Dear Aarti Bains ,

Thank you so much for your suggestion!

It depends a lot on the fungi you want to preserve, not all of them can withstand cryopreservation. In our laboratory we have used Fusarium cryopreservation in 20% glycerol and the sterile filter paper method for fungi such as Exserohilum and Bipolaris.

I would recommend using more than one preservation method and evaluate viability after a few days or weeks.

Thank you so much Karla Y. Leyva-Madrigal ! You made a good point! Is it possible for you to share the sterile filter paper method protocol if there are any? Thank you again!