I want to extract gangliosides from piglet's brain after euthanizing them. I will not extract gangliosides right away but after within 1 month of euthanizing date I want to start the extraction process.
Freeze them with/in liquid nitrogen and/or putting in a -80 deg Celsius freezer for preventing lipid oxidation.
I've selected an interpretivist philosophy along with a qualitative inductive approach for my research. I plan to employ semi-structured interviews as my primary data collection method. However, I...
12 May 2024 7,816 4 View
Certainly! Here's a refined version of your question: "I've been encountering challenges downloading and reading journal articles. Despite some being accessible through Sci-Hub using their DOI...
12 April 2024 8,975 5 View
I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...
19 April 2022 6,297 3 View
I am going to extract RNA from boar semen samples. I get low concentration of RNA and I wanted to know minimum RNA concentration required to prepare cDNA and do RT-PCR
06 April 2022 7,031 3 View
can we apply ARDL method ,after adding moderated variable into the model, because my sample size is 30 ,and variables are mixed of I(0) and I(0).
23 January 2022 9,508 3 View
I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...
11 May 2021 8,362 8 View
I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...
22 April 2021 3,911 3 View
I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.
28 March 2021 7,871 6 View
Hello, I want to know the best method that we can use to differentiate THP-1 cells into immature dendritic cells please is anyone there who can help with this?
01 January 1970 5,155 0 View
Dear colleges, i hope to use the ARDL model to examine the impact of FDI on Innovation.All variables are in ratio except the dependent variable. so can I employ these data to run the ARDL model as...
01 January 1970 10,037 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View