Which amount of RNA concentration required for cDNA synthesis from sperm samples?

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Where does phenomenological research fit within the layers or stages of the research onion proposed by Saunders et al. (2018)?

I've selected an interpretivist philosophy along with a qualitative inductive approach for my research. I plan to employ semi-structured interviews as my primary data collection method. However, I...

12 May 2024 7,816 4 View

Which site can assist me to download article easily?

Certainly! Here's a refined version of your question: "I've been encountering challenges downloading and reading journal articles. Despite some being accessible through Sci-Hub using their DOI...

12 April 2024 8,975 5 View

How can I extract RNA from commercially available semen samples of boars?

I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...

19 April 2022 6,296 3 View

What is the best method for evaluating a moderate role of a variable in a time series analysis?

can we apply ARDL method ,after adding moderated variable into the model, because my sample size is 30 ,and variables are mixed of I(0) and I(0).

23 January 2022 9,508 3 View

I did SOD assay tissue samples. I get negative values for the concentration of the samples. Why is this happening?

I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...

11 May 2021 8,361 8 View

In Bradford assay with BSA, standard and samples show OD values but in concentration table , samples' concentration showed as '0'. Possible reasons?

I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...

22 April 2021 3,910 3 View

Do I need to determine the protein concentration before conduct GSSG/GSH, SOD, MDA assays?

I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.

28 March 2021 7,870 6 View

THP-1 cell differentiation in to immature dendritic cells

Hello, I want to know the best method that we can use to differentiate THP-1 cells into immature dendritic cells please is anyone there who can help with this?

01 January 1970 5,154 0 View

Is ot possible to run a ARDL model

Dear colleges, i hope to use the ARDL model to examine the impact of FDI on Innovation.All variables are in ratio except the dependent variable. so can I employ these data to run the ARDL model as...

01 January 1970 10,037 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,881 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,621 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View