Dilki Adikari

5 Questions 3 Answers 0 Followers

Questions related from Dilki Adikari

How can I extract RNA from commercially available semen samples of boars?

I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...

20 April 2022 6,297 3 View

Which amount of RNA concentration required for cDNA synthesis from sperm samples?

I am going to extract RNA from boar semen samples. I get low concentration of RNA and I wanted to know minimum RNA concentration required to prepare cDNA and do RT-PCR

07 April 2022 7,031 3 View

I did SOD assay tissue samples. I get negative values for the concentration of the samples. Why is this happening?

I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...

12 May 2021 8,362 8 View

In Bradford assay with BSA, standard and samples show OD values but in concentration table , samples' concentration showed as '0'. Possible reasons?

I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...

23 April 2021 3,911 3 View

Do I need to determine the protein concentration before conduct GSSG/GSH, SOD, MDA assays?

I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.

29 March 2021 7,871 6 View