Dilki Adikari

7 Questions 3 Answers 0 Followers

Questions related from Dilki Adikari

What sort of biomarkers that I can use in IHC for pig brain to detect learning and memory?

I'm going to do IHC in pig brain to detect biomarkers related learning and memory. It's very hard to find biomarkers for pig model. I would like to have some suggestions from fellow researchers...

13 March 2025 966 0 View

How to preserve and store 30 days old piglet's brain to extract gangliosides later on?

I want to extract gangliosides from piglet's brain after euthanizing them. I will not extract gangliosides right away but after within 1 month of euthanizing date I want to start the extraction...

20 December 2023 4,805 1 View

How can I extract RNA from commercially available semen samples of boars?

I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...

20 April 2022 6,326 3 View

Which amount of RNA concentration required for cDNA synthesis from sperm samples?

I am going to extract RNA from boar semen samples. I get low concentration of RNA and I wanted to know minimum RNA concentration required to prepare cDNA and do RT-PCR

07 April 2022 7,059 3 View

I did SOD assay tissue samples. I get negative values for the concentration of the samples. Why is this happening?

I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...

12 May 2021 8,393 8 View

In Bradford assay with BSA, standard and samples show OD values but in concentration table , samples' concentration showed as '0'. Possible reasons?

I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...

23 April 2021 3,945 3 View

Do I need to determine the protein concentration before conduct GSSG/GSH, SOD, MDA assays?

I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.

29 March 2021 7,899 6 View