Hello, I want to know the best method that we can use to differentiate THP-1 cells into immature dendritic cells please is anyone there who can help with this?
Certainly! Here's a refined version of your question: "I've been encountering challenges downloading and reading journal articles. Despite some being accessible through Sci-Hub using their DOI...
12 April 2024 8,975 5 View
I am trying to run dioxin and furans on a XEVO-TQ-XS in APGC mode and see most frequent ion abundance ratio failures on low level standards. The same analysis has been performed on the same...
17 January 2024 7,340 0 View
I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...
19 April 2022 6,297 3 View
I am going to extract RNA from boar semen samples. I get low concentration of RNA and I wanted to know minimum RNA concentration required to prepare cDNA and do RT-PCR
06 April 2022 7,031 3 View
can we apply ARDL method ,after adding moderated variable into the model, because my sample size is 30 ,and variables are mixed of I(0) and I(0).
23 January 2022 9,508 3 View
Can any one suggested an author processing fees free research journal for publishing the algae related work...The journal should not be a predatory one. Example: comparative study on algal...
05 December 2021 6,611 4 View
I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...
11 May 2021 8,362 8 View
I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...
22 April 2021 3,911 3 View
I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.
28 March 2021 7,871 6 View
I have two data sets which inform of lines. Empirical data of human movement and software generated human movement. I want compare both to find out the similarity level. please suggest me software...
22 April 2019 8,124 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 640 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,892 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View