How to prepare the standard for HPLC to determine sulphur containing amino acid?

More Sheik Md Moniruzzaman's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Bangladesh government's reported plan to use lethal force against protesters? We need help Urgently ?

Please Help Us Urgently

01 August 2024 711 1 View

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students ?

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...

29 July 2024 2,181 7 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

How to calculate pseudo order kinetics?

I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...

21 July 2024 8,807 6 View

How can I calculate spin texture using Quantum Espresso for non-colinear case ?

I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??

19 July 2024 8,571 1 View

What is the average energy consumption per gate operation with superconducting qubit?

Could you provide information on the average energy consumption per gate operation when using superconducting qubits, specifically focusing on the microwave power required to implement gates? I...

15 July 2024 954 0 View

What is the Scopus and Beall's dilemma?

I've found that some journals are both Scopus-indexed and listed on Beall's list as predatory or potentially predatory. Why does this discrepancy occur? Are there any more reliable platforms than...

12 July 2024 5,158 1 View

Is there any gene present in our body that did not came from our parents?

All I want to know is whether it is feasible or not. How did genes come to be if they do not come from parents?

12 July 2024 7,221 1 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

Issues in coating of cathode material on Al foil, why we are not getting proper coating and results when the cathode was coated on Al-foil..?

We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...

09 July 2024 5,123 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

How to know if the oil palm tree is functioning properly in terms of absorption of different nutrient?

The idea is to design an IoT system that is able to capture real-time conditions from an oil palm tree to ensure optimal growth. Nutrients such as Sulfur, Nitrogen, Boron, Zinc, Copper and Iron.

30 June 2024 3,046 2 View

Regarding first author name in articles ?

Dear Sir, Four publications in my profile do not belongs to me belongs to MALLAMMA, University of Mysore , Mysore: They are 1. Kinetic and Mechanistics studies of Pd(II)...

29 June 2024 1,732 0 View

How to distinguish the origin of sulfides in subduction zone interface rocks?

If we find sulfides in subduction zone interface rocks, how can we distinguish whether these sulfides are related to crust-derived fluids or originate from the original mantle wedge? Especially...

15 June 2024 654 1 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

How can I clean autoclave (PTFE) from sulfur contamination ? in all aspect of color, smell and existence of Nano particle of sulfur??

the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...

31 May 2024 2,985 0 View

Bruce Neagle

Just weigh accurately on an analytical balance ~10 mg of the peptide and dissolve it in 100 mL of HPLC solvent. Nominal concentration is 100 ug/mL not including moisture.

Thus ug/mL S = (1000 ug/mg x mg/100 mL) x (MW Sulphur/MW Cysteine) x (100.0-% Water/100.0)

Sheik Md Moniruzzaman

Thanks a lot for your information. Best regards,