How to prepare the purified growth hormone protein to pharmaceutical form?

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Domenico Sanfelice

You can read this paper to find the answer to your question, it is free!

http://www.ncbi.nlm.nih.gov/pubmed/23409149

Lawrence Kirk Allin

I am assuming you want to make an inject-able form. Check purity, and indotoxin levels. I am not sure what indotoxin level will not cause shock it is low. Once you have pure protein. You generally need to polish your protein on a Q matrix to remove indotoxins. Tangential flow filtration into sterile PBS, sterile filter. Everything should be done in a clean room, with proper monitoring, under aseptic conditions. Everything cleaned with 1 M NaOH for a minimum of 30 minuets. If you can not a hood will work. I would be very careful if you are thinking about putting this in anyone.

Waleid Shakweer

I will produce bovine growth hormone for animal injection not for human