The bioactive compound to be encapsulated is soluble in water but not in oil and the lipoid s75 to be used is not pure phospholipids, do we have to consider only its total phospholipids content to prepare a working concentration.
Generally you must first dissolve your lipids(all hydrophobic components) in chloroform an then evaporate it using a rotavac creating a thin layer. Then hydrate your lipid layer with a mixture of DI water and your bioactive compound which you want to encapsulate inside the liposome.
Hi, I would just add one thing that use ethanol as compared to chloroform because ethanol is less expensive and less toxic. See the following paper for the preparation;
https://www.researchgate.net/publication/282812596_Proliposome_Powders_Prepared_Using_A_Slurry_Method_For_The_Generation_of_Beclometasone_Dipropionate_Liposomes
Article Proliposome Powders Prepared Using A Slurry Method For The G...
You can dissolve your active in the hydration media that is going to be used wht you disperse you lipid film (Bangham method).
If your drug is hydrophilic then you dissolve it in aqueous medium or hydration medium, for dispersion of lipid film in the round bottom flask, you will use the same aqueous or hydration medium.
in case if your drug is lipophilic then you dissolve the drug in organic solvent followed by the addition of lipid. Rotary evaporator is then used to evaporate the organic solvent and leaving a lipid film in the round bottom flask, and then aqueous medium or hydration medium is used to disperse the lipid film, resulting is the formation of liposomes.
Hi,
Yes, total lipid content would be fine. Depending on your application, you can make liposomes from 0.2 to 40 w/w percent.
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