Hey arpita,

I have the same problem. The main trouble is, that cuprizone seems to be inactivated at certain temperature (which exactly is not known).

Do you know, if there is a possibility to produce cold-pressed pellets?

some people use carboxy methyl cellulose (CMC) as binding agent and then autoclave pellets for high fat diet preparation but then as you said I was not sure if cuprizone diet can be autoclaved and hence i mixed powder of pellet + cuprizone as diet.( did not make pellets basically).

Feel free to ask if any further questions you have.

-Arpita