How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

I teach a STEM career course for IT majors at a small liberal arts school. I am looking for collaborators/information sources for a new book ?

Looking for collaborators/info sources for a new book I am writing in the career/life coaching field on "Adult Bullies". Most research has been done on childhood bullying, however they eventually...

27 November 2020 6,532 1 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

Is annealing should be done again after a year of nano-particles fabrication and they are found to be agglomerated?

I have prepared CeO2 based nano-particles. due to some technical issues some of the properties like electronic structure and magnetic properties are pending. the samples were prepared...

08 October 2020 769 3 View

Advice writing an unbiased qualitative research question?

Hi, I was wondering whether kindly souls out there might be able to help me with some advice regarding the formulation of a RQ for my qualitative study please? I'm trying to refine an early...

25 March 2020 6,512 5 View

Is there any python code which converts text(unstandarized) into a csv fom?

I have an unstandarized text data . So when I convert the text into csv form it results are not into a proper csv format.

05 February 2020 4,556 3 View

Do you think the word/phrase "Economic Growth" is an innocent and positive one?

15 January 2020 2,890 14 View

What ML/AI algorithms can I use to analyze Bank statement and detect frauds??

Ml algorithms which can be useful to analyze bank statement and then extract information about that frauds.

08 January 2020 9,890 6 View

Any free software to convert my audio interview to text ?

I am doing Qualitative Research

31 December 2018 4,092 8 View

What are barnacles environmental constraints ?

I've got some data on barnacles across a shore line and was after a guide to the appropriate review that discuses the biochemical changes within barnacles during desiccation. Is there one that...

03 December 2018 7,280 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View