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Questions related from Ryan Mohd
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
03 May 2024 4,817 0 View
I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...
29 February 2024 153 2 View
Hello All, I have several questions about qPCR. I will explain what I have done to give you a clear picture. I have 2 kidney samples. 1 wildtype and 1 KO for a specific gene. We have extracted...
26 February 2024 6,314 2 View
I have extracted genomic DNA from 2 tissues. 1 wild type and 1 KO. I havd designed primers that should amplify a region in between the two loxp sites. I want to validate the effectiveness of the...
23 February 2024 9,645 0 View
I have raw qPCR data. 2 samples "1 control and 1 with gene knock out". I made a serial dilution of each sample and performed a qPCR using 2 reference genes and 3 genes of interest. Now I have the...
22 February 2024 2,726 5 View
Is there a protocol to sort proximal tubule cells after Cre loxp recombination and to sort the rocombined vs the WT cells to then perform qPCR to approve that the cre lox recombination actually...
12 October 2023 6,827 0 View
1- After deparaffinization, do I rinse my slides with proteinase K buffer only then with the buffer + proteinase k (1ml TRIS 50mM EDTA 1 mM + 1 ul prot. k) ? or just add the buffer + prot K...
20 June 2023 6,848 0 View
