I am intrested how to prepare 5' labeled primers (6-FAM, NED and HEX)? Specifically, do this primers that come in powder form need to activate for some time after I disolved them before I prepare working solutions out of them?
Just treat them the same as unlabelled primers....dissolve in TE but storage is slightly different as labelled primers are slightly less stable to freeze thaw and light so I would prepare aliquots to avoid too many freeze thaw cycles and store in a light proof box or dark tubes but they are just as soluble as unlabelled primers so can be used as soon as they are dissolved