What should be the typical yield of cDNA after following RT PCR in ARCTIC protocol for sars cov 2 sequencing by nanopore ?

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How to calculate work function of an interface in SIESTA using MacroAve?

I am trying to compute work function of various interfaces using SIESTA. I am not being able to determine the limits of the step (filter) function, required for the inputs of MacroAve. Also,...

28 November 2019 7,735 0 View

Getting permission to use modified kuppuswami scale 2018?

I am doing research on prevalence of anemia among adolescent girls and i want to categorise girls based on their income so please help to get right to use this scale

12 April 2019 8,089 0 View

How to interpret ph.out file in quantum espresso?

Hello, I am trying to calculate properties of crystal using quantum espresso with winmostar. I am not able to interpret the output file and also draw the phonon band structure. Please guide me...

18 July 2018 9,429 2 View

Is there any natural substance that i could add to my home-made nettle fertilizer to make it less smelly?

The nitrogen in my home-made fertilizer made of nettle is mostly in the form of ammonia and has a horrible smell. Is there any natural and ecological way to trap the ammonia?

09 May 2018 7,894 11 View

Is total gram quantity or concentration of active ingredient in pesticides more important for pest control?

I want to know more about the insecticidal application strategies as I' m going to compare different application strategies for the management of insect pests infesting okra. So give idea in...

31 March 2015 5,255 7 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Hi all, what is the interpretation of the results of this gel electrophoresis image?

We aim to measure the RNA expression of p53 and K-RAS biomarkers in colon cancer samples. We extracted the RNA from Rneasy FFPE kit and converted the it to cDNA and did real-time PCR using Cyber...

08 July 2024 7,781 6 View

How to properly dilute RNA for cDNA?

Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...

07 July 2024 9,206 4 View

Sybergreen qpcr troublshoulting?

500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...

06 July 2024 5,478 4 View

Why is my HPRT1 housekeeping gene not coming at same Ct in qPCR?

I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...

04 July 2024 5,605 3 View

How to amplify NCF1 with real time PCR without amplifying its pseudogenes NCF1B, NCF1C ?

I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !

04 July 2024 3,068 3 View

CCN concentration over Arctic region?

I need CCN concentration over Arctic region, any observational dataset is welcomed. Thank you.

01 July 2024 6,082 0 View