how to prepare 30 mM of acetate buffer solution ( Sodium acetate + Glacial acetic acid)?
Make a solution of 30mM of sodium acetate, make a solution of acetic acid (molar concentration of glacial acetic acid is 17.4). if you need a basic buffer, adjust the pH by adding the 30mM acetic acid into the sodium acetate solution, for a acidic buffer, adjust pH by adding sodium acetate into 30mM acetic acid solution.
Another way is to make 2 solutions: 30 mM sodium acetate and 30 mM acetic acid. Mix them together while monitoring the pH to get the desired pH. By doing it that way, no adjustment of the final volume is needed.
Or you can use published recipes, such as these, for 0.1 M buffer:
http://delloyd.50megs.com/moreinfo/buffers2.html#acetate
The pH may change slightly when you dilute to 30 mM.
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