Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

What is the first thing that comes to your mind when you think of the effectiveness of foreign aid on development? Effective/not effective, why?

I'm currently doing a research project on the effectiveness of U.S. aid in Georgia, and would very much appreciate your insights.

22 December 2020 4,189 1 View

Can peptides survive high temperatures?

I'm looking for ways to functionalise a material with a cell-adhesive peptide (relatively short chain - 6aa) and I was just wondering if peptides can withstand high temperatures (100-150 C) for 30...

15 January 2020 8,978 4 View

Abnormally high fluorescence read with qpcr, and no amplification showing on agarose gel, what could be going on?

Hi! I've been running qPCR on some drinking water samples with a primer/ probe FAM assay. In any given run, there have been at least a few samples with artificially high fluorescence reads,...

16 October 2019 8,060 3 View

How to prepare agar amended with mercuric chloride?

Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some...

17 June 2019 5,047 5 View

Interview guides for UTAUT?

30 January 2019 5,443 4 View

How much does our childhood affect the way we think and make investment choices?

Subconscious, subjective drivers.

22 January 2019 5,397 5 View

ReNcell VM cell culture problems - why are they dying after I split them?

The cells look healthy after thawing, but they die when I try to split them. I have tried making fresh media and fresh matrigel, and have tried filtered and non-filtered Accutase. Does anyone have...

19 December 2018 630 9 View

Is it necessary to dechorionate zebrafish embryos to extract genomic DNA and RNA ?

I tried to dechorionate embryos with pronase at 1mg/mL. I made 3 washes but I obtained a high mortality rate and the medium was dirty. Thank you for your answers

03 January 2017 3,455 4 View

Can you help me with Activity measure of trypsin?

Sustrate=BAPNA I get a white solution when i mixed BAPNA(with DMSO) and buffer Tris-HCL/CaCl2 First 3 minutes the activity goes down, after 3 min stars to go up but slowly. Is this normal?

25 July 2016 6,718 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View