Success in simultaneous isolation of DNA and RNA from the same PAXgene Blood RNA tube?

Sophia Horigan @Sophia-Horigan

14 October 2023 1 7K Report

And is there any insight if DNA methylation would be impacted?

Péter Gyarmati

The second question is easier, and the answer seems to be no

Article Exploring the influence from whole blood DNA extraction meth...

As for simultaneous extraction of RNA and DNA from blood, I am not aware of any convincing kits/protocols

(eg

Article Two hours method for RNA and DNA co-extraction from blood of...

)

and especially there is no comparison/standardization of any sort. If you can, isolate RNA/DNA separately; if not, can look at the Qiagen Allprep kits and experiment with blood or isolate total nucleic acid and treat w RNAse/DNAse.

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