I want to quantify the amount of Ru that are bound to a peptide, and my plan is to use ICM-MS or AA to get this data. I already know that my Ru complex binds to this peptide, and the peptide precipitates over time, so my plan is to centrifuge my solution in order to isolate the precipitate (peptide + complex). From there, I need to perform the acid digestion using HNO3 and heat, is that right? Should I dry my precipitate completely before adding the acid? Do I need to use ultra pure HNO3 for both ICP-MS and AA analysis? After the digestion is done, do I need to add more buffer to the solution or it is ready to be injected into the equipment?
Thank you very much
Please look at the following below links which may help you in your analysis:
http://downloads.hindawi.com/archive/2013/851713.pdf
https://www.epa.gov/sites/production/files/2015-12/documents/3050b.pdf
Thanks
Hi Janaina
Ru is fairly volatile as RuO4, so oxidative conditions should be avoided. If your method requires oxidation and acid media use a closed vessel digestion with HCl as complexing agent to keep Ru in solution. You will probably not need ultra high purity reagents because Ru is not abundant in the environment. Samples and standards should ideally have the same composition with respect to acid/buffer. Use a suitable internal standard for ICP-MS.
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