How to predict if specific protein can bind to another specific protein?

01 January 2016 1 4K Report

I have two specific proteins in mind and before i go to do Co-IP, i want to check if there is any prediction bioinformatic tool that i can enter my two protein names and this tool can tell me if they could bind or not?

Steingrimur Stefansson

Hi Hatem, there are databases of protein-protein interactions:

http://www.bindingdb.org/bind/ByTargetNames.jsp

http://string-db.org/

http://pisite.hgc.jp/

Badges
Science topic

More Hatem A Abou-Ouf's questions See All

What is the ideal time to administer prophylactic antibiotics to cancer patients after anticancer therapy?

I need clarification on the appropriate timing for administering prophylactic antibiotics to cancer patients.

29 April 2024 7,106 2 View

Anyone working on Sustainable University Campus Design and Planning ?

I would like to connect with colleagues working on sustainable university campus design. please tell me the stage of your research development and a little about the specific point of interest.

27 January 2024 3,285 0 View

How can I get a list of miRNAs on a chromosomal band?

Hi, I need to get a list of miRNAs that located on certain chromosomal bands. If there is any bioinformatic tool or website where I can find this information, please share. Thanks

12 October 2015 5,218 3 View

How can I get a list of miRNAs on a chromosomal band?

Hi, I need to get a list of miRNAs that located on certain chromosomal bands. If there is any bioinformatic tool or website where I can find this information, please share. Thanks

09 October 2015 3,123 4 View

How can I get the chromosomal location of a list of genes?

I am looking for a bioinformatic website or tool to get the locations of a list of genes but in one shot instead of doing it gene by gene. Is there a way to do this? Also, if you have R script to...

04 May 2015 5,348 17 View

How can I get the chromosomal location of a list of genes?

I am looking for a bioinformatic website or tool to get the locations of a list of genes but in one shot instead of doing it gene by gene. Is there a way to do this? Also, if you have R script to...

03 May 2015 9,441 19 View

Any advice on prostate Cancer cell lines, genome portal?

Hi, I am looking for a source to get copy number data for prostate cancer cell lines such as PC3, LNCaP, DU145 , ..etc. I checked Oncomine but i can not find these data. Share plz if you have a...

17 January 2015 7,901 2 View

Any advice on prostate Cancer cell lines, genome portal?

Hi, I am looking for a source to get copy number data for prostate cancer cell lines such as PC3, LNCaP, DU145 , ..etc. I checked Oncomine but i can not find these data. Share plz if you have a...

31 December 2014 3,686 2 View

What are the best housekeeping control genes for Prostate Cancer using FFPE samples?

Hi everyone, I isolated RNA from FFPE PCa samples with very acceptable quality. CT values are very consistent. No more than 0.5 CT difference and all under 34. Now, i am selecting the best...

27 November 2014 1,199 2 View

What genes are shown by qPCR using RNA isolated from FFPE ?

Hi, My method for isolating RNA from FFPE samples needs some tweaking and currently I am getting consistent Ct values among technical replicates. Most values are within acceptable range (28-32)....

10 November 2014 4,164 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

I want to conduct a research using quasi experimental design to test two treatment and one control what type of statistical tools do l use ?

What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks

18 July 2024 3,581 3 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Which research tool for expert validation for our study?

I am a 3rd year Computer Science student currently writing our Bachelor's thesis about finding diverse k-shortest paths in pedestrian networks. We have chosen 3 local areas as our proposed...

15 July 2024 4,289 0 View