We have extracted minute amounts of proteins with SDS and now want to precipitate the proteins for analysis. We already tried cold acetone, TCA etc but nothing worked.
Any ideas are welcome.
Thanks
I also had the same problem. The solution was dialyze the samples, and also gives lyophilized. You can try to change the form of quantification of proteins. In my case I use my samples without precipitating as it is for western blot. I hope this helps.
You might still have SDS in your protein samples even after dialysis. I've read varying accounts of that problem even after extraction. If you don't care about the SDS, then it shouldn't be a problem. If you want the SDS out, I think Pierce has a kit for small volumes.
Best of luck!
I do not mind SDS-complexed with protein but want to remove excess SDS from protein-SDS complex. Somebody told me that SDS can be readily ppt by KCl as potassium salt of DS is insoluble. However, I have never tried it. Thanks for all these ideas. Any reference for 90% cold ethanol ppt?
Never tried it like that, but i think 50 % methanal, 10 % acetate soln in water may work....try this
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