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Questions related from Tiane Finimundy
I'm having problems detecting protein by western blot, using chemiluminescence. The bands are appearing very weak, I have since modified some details but this does not change. Lysis buffer is...
07 July 2012 4,309 35 View
Can someone tell me if in the detection phase of the WB I can use a fluorochromo such as FITC or Sybr green instead of the kit with ECL detection on film? The visualization of the bands would...
07 July 2012 4,124 4 View
Can SYBR Green be used in the detection of proteins by western blot, instead of using chemiluminescent detection? Someone else suggested fluorochrome, while FITC worked.
07 July 2012 7,396 0 View