Hello. I want to check the expression pattern of a particular protein after transfecting it into S2 cells and then performing whole cell extraction. I need some suggestions regarding how to perform whole cell extraction using S2 cells. Previously I tried just to lysate the cells using 4X sample buffer and then boil it at 98 degree celsius for 5 minutes. It seemed that the expected band is not so prominent as I hoped it to be.
Any suggestion would be very helpful. Thanks in advance.
Hello
look at links here will help you
Good Luck
http://genesdev.cshlp.org/content/20/21/2985.long
http://www.virologyj.com/content/10/1/210
http://www.flyrnai.org/DRSC-PRC.html
http://www.ijbs.com/v08p0606.htm
http://jcs.biologists.org/content/114/11/2015
http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1000320
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