Zahid Sadek Chowdhury

6 Questions 3 Answers 0 Followers

Questions related from Zahid Sadek Chowdhury

What is the consequence if there is a premature stop codon in the tagged portion of a protein?

Hello all, I have constructed a plasmid which expresses my protein of interest in S2 cells. I have tagged the protein on its N-terminal with FLAG and on C terminal with V5 epitope. The protein...

11 November 2017 5,659 3 View

How to measure an mRNA level of a gene by qPCR?

Hello everyone, I want to measure the expression level of my gene of interest though qPCR analysis upon knocking it down by RNAi interference in cultured cells. As far as I understood, the basic...

08 August 2017 2,824 5 View

How to perform whole cell extraction for Drosophila S2 cells?

Hello. I want to check the expression pattern of a particular protein after transfecting it into S2 cells and then performing whole cell extraction. I need some suggestions regarding how to...

10 October 2015 8,387 1 View

How do I getting amplification in my PCR?

Hi everyone, I am having trouble in getting band with PCR. My forward primer is an oligonucleotide having 70 base pairs with Tm of 80.4 degree and the other one (Reverse primer) is of 38 base...

06 June 2015 5,407 7 View

How can I tackle troubleshooting in plasmid design?

Hi all. I designed a plasmid construct inserting a gene about 5.5kb long. After sequencing, I am getting a 18 base deletion around the 3 prime region of the construct. I have tried it many times...

06 June 2015 6,331 2 View

Why did my S2 cell culture turn yellow after transfection with pMT vector and using CuSO4?

Hello all, I am using S2 cell line to transfect a pMT construct to check the expression pattern. After induction of expression with CuSO4 addition my cultures became yellow after 12 hours and i...

01 January 2015 3,270 4 View