What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Why differs CT values of one sample between its triplos (Taqman Assays and qPCR)?

At first, we performed transfections. To analyze that transfection, we performed RNA isolation, cDNA synthesis and qPCR. Using the qPCR, we analyze multiple samples, whereof each sample we...

19 March 2020 2,301 4 View

What is the optimal method to the cryopreservation of SH-SY5Y cells?

I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...

14 November 2019 3,474 3 View

What data analysis tools can be used in order to validate data quality?

I am looking for data analysis tools that can be added into a new database (legacy system migration, without database from legacy system) which takes structured data (pre-determined format, seen...

25 March 2018 269 3 View

What should I do when comparing response frequencies in multiple response items?

I have a multiple response item consisting of 10 response options. Participants were instructed to choose 3 of them. I want to know which one of those response options was picked the most. Of...

06 December 2017 6,047 6 View

How to reduce off-target effects siRNA?

28 November 2016 2,726 8 View

How to incorporate thymidine analogs by PCR?

I am aiming to create 5 - 10 kb amplicons by PCR with all thymidines replaced by different analogs (i.e. BrdU, EdU, CldU, IdU). For this, I tried to simply replace dTTP by dBrdU (or other) in the...

29 June 2016 9,892 1 View

What are good alternatives for rhizon samplers to sample pore water from heavy clay sediments?

11 April 2016 3,949 3 View

Should I use an acid or a base as stop solution at the end of an ELISA?

07 October 2014 3,475 7 View

Are there Pollen datasets for your country to test its inverse effect on Covid-19/flu-like incidence?

We recently found - using Dutch multi-year datasets - that pollen are likely the seasonal factor in inhibiting flu-like epidemics (see below). We tested it for 2016-2019 and will expand that...

01 January 1970 7,338 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View