Hello,
What type of statistical test would one use to find a significant difference in antigen expression? (Ex: Surface protein expressed in cancer cells vs. non-cancer cells) would one perform statistical analysis in the flowjo software, excel, or prism? still very new to this.
thanks,
Andrew
Dear Andrew Kim
You should use FlowJo software to analyze your flow cytometry datas. There are so many useful videos on youtube for this program. After that you can use Prism GraphPad software to make charts and find the statistics.
Good luck.
Hello, depending on the data set that you have, I will suggest to use T statistics or bayesian statistics.
For protein expression level, first get the median fluorescent intensity from your cell population positive for that marker. Then you can analyse your data on Excel or Prism. The statistical test you choose will depend on the number of parameter in your experiment.
When comparing antigen expression between two groups (e.g., surface protein expression in cancer cells vs. non-cancer cells), you would typically use a statistical test to determine if there is a significant difference. The choice of statistical test depends on the specific characteristics of your data, such as sample size, distribution, and the type of comparison you want to make. Here are a few commonly used tests for this type of analysis:
Regarding the software choice for statistical analysis, both FlowJo and Excel are primarily used for data visualization and basic analysis. For more advanced statistical analyses, such as the tests mentioned above, you would typically use dedicated statistical software such as GraphPad Prism, SPSS, or R. These software packages offer a wide range of statistical tests and provide more robust and comprehensive statistical analysis capabilities.
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