18 Questions 18 Answers 0 Followers
Questions related from Andrew Kim
Hello all, I am a 4th year grad student in the proteomics field. so here's my situation, a predecessor of mine did some MS analysis on mouse EAE models and I "inherited" if you will, the RAW...
24 April 2025 5,029 0 View
Hello, I'm working with data from mouse brain tissue that was analyzed with mass spectrometry. This data was from nearly 2 years ago and its annoying that many of the UniProt accessions dont...
21 March 2025 974 1 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
12 July 2024 8,753 0 View
Hello, So, I am analyzing serum proteomics with MS from autism mouse models and trying to compare that to human serum data. So its: Differentially expressed proteins in human serum vs....
10 July 2024 7,175 7 View
Hello, I am not familiar with different analysis modes of mass spectrometry so I seek your help. I wish to understand the difference between DIA and DDA analysis. I understand that DDA searches...
16 August 2023 10,009 8 View
Hello, I am currently working on a project of comparing blood samples of children with ADHD and ASD. We plan to do the following: 1. receive blood from the hospital of control blood, ADHD blood,...
14 July 2023 1,548 5 View
Hello, I am planning on doing a TMT-labeled MS analysis experiment for global proteomics of glioma primary stem cells. Its my first time doing the experiment so Im referencing the protocols of my...
23 June 2023 9,270 0 View
Hello, What type of statistical test would one use to find a significant difference in antigen expression? (Ex: Surface protein expressed in cancer cells vs. non-cancer cells) would one perform...
28 February 2023 2,214 4 View
I know this is a strange question, but I accidentally made a stock for Western Blot (TBST and BSA) with an antibody that cannot be used for WB. And someone in my lab said that as long as TBST...
14 February 2023 10,023 1 View
Hello, Our lab mixes primary antibodies with BSA and Sodium Azide and I wonder what purpose BSA serves. I understand that BSA is used for blocking buffers. But the membrane is already blocked...
09 February 2023 6,995 6 View
Hello, I was trying to probe for an integrin subunit that was supposed to be apparent at 100 kDa and I have two questions; 1. when I tried the first time, the bands showed up at around merely 70...
06 February 2023 1,204 17 View
Hello, I am wondering if cell doubling times are universal or do they depend on factors such as plate / flask size? I ask because I've searched some journal articles differ in doubling time of...
16 December 2022 6,326 3 View
Hello all, One of my supervisors told me that when transfecting HEK293FT cells, the P3000 reagent is not really necessary. But in other thermo fisher protocols, it says to use the P3000 reagent...
05 December 2022 2,104 5 View
when it comes to transfection of Lentivirus, why is Lipofectamine 3000 better? Whats the difference? Or is it just marketing? Additionally, how does Lipofectamine and fugene differ?
04 December 2022 627 0 View
Hello, I have a question on TMT labeling MS. Why is it that we randomize our samples (we randomize with an excel function) for TMT labeling? Much appreciated, Andrew
16 November 2022 3,905 0 View
Hi, I'm using the alamarBlue reagent to continue testing small-molecule drugs on glioblastoma cells. I seed 62500 cells on 96 well plates. I draw a standard curve first. I go from 500000 cells...
26 October 2022 2,825 0 View
This is just a simple definition/jargon question but why are fluorescent probes called two different names? For example why is GFP called GFP and Alexa Fluor 488?
11 October 2022 3,871 3 View
Hello world, I am currently doing an experiment with glioblastoma cells and cell killing using a small molecule drug. Here's how my supervisor told me to do it. 1. Serial dilute one 100 pi...
04 October 2022 4,585 0 View
