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Questions related from Andrew Kim
Hello, I am planning on doing a TMT-labeled MS analysis experiment for global proteomics of glioma primary stem cells. Its my first time doing the experiment so Im referencing the protocols of my...
23 June 2023 9,214 0 View
Hello, What type of statistical test would one use to find a significant difference in antigen expression? (Ex: Surface protein expressed in cancer cells vs. non-cancer cells) would one perform...
28 February 2023 2,143 4 View
I know this is a strange question, but I accidentally made a stock for Western Blot (TBST and BSA) with an antibody that cannot be used for WB. And someone in my lab said that as long as TBST...
14 February 2023 9,954 1 View
Hello, Our lab mixes primary antibodies with BSA and Sodium Azide and I wonder what purpose BSA serves. I understand that BSA is used for blocking buffers. But the membrane is already blocked...
09 February 2023 6,947 6 View
Hello, I was trying to probe for an integrin subunit that was supposed to be apparent at 100 kDa and I have two questions; 1. when I tried the first time, the bands showed up at around merely 70...
06 February 2023 1,123 17 View
Hello, I am wondering if cell doubling times are universal or do they depend on factors such as plate / flask size? I ask because I've searched some journal articles differ in doubling time of...
16 December 2022 6,222 3 View
Hello all, One of my supervisors told me that when transfecting HEK293FT cells, the P3000 reagent is not really necessary. But in other thermo fisher protocols, it says to use the P3000 reagent...
05 December 2022 2,036 5 View
when it comes to transfection of Lentivirus, why is Lipofectamine 3000 better? Whats the difference? Or is it just marketing? Additionally, how does Lipofectamine and fugene differ?
04 December 2022 553 0 View
Hello, I have a question on TMT labeling MS. Why is it that we randomize our samples (we randomize with an excel function) for TMT labeling? Much appreciated, Andrew
16 November 2022 3,820 0 View
Hi, I'm using the alamarBlue reagent to continue testing small-molecule drugs on glioblastoma cells. I seed 62500 cells on 96 well plates. I draw a standard curve first. I go from 500000 cells...
26 October 2022 2,680 0 View
This is just a simple definition/jargon question but why are fluorescent probes called two different names? For example why is GFP called GFP and Alexa Fluor 488?
11 October 2022 3,804 3 View
Hello world, I am currently doing an experiment with glioblastoma cells and cell killing using a small molecule drug. Here's how my supervisor told me to do it. 1. Serial dilute one 100 pi...
04 October 2022 4,506 0 View
