I am trying perform esterase assay for my fungal isolates directly. I am looking for a complete protocol. Moreover, Is it necessary to pure the enzyme first or we can perform it on our isolates directly too?
I want to increase the number of conformations which is by default 10. I want to get 100 conformations of a single ligand. Is it possible using autodock vina or any other software?
11 April 2024 8,736 2 View
Hello there! I am trying to dock ZIF-8 (MOF) with the drug doxorubicin using PyRx and discovery studio for visualization of results. My queries are: 1. When I convert .cif file to .pdb using...
27 March 2024 2,007 4 View
Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites
08 October 2023 6,968 1 View
I have data in the form of graphs and peaks and I want to find Tm and Tg from DSC/TGA graphs I have. Similarly, I have to perform ANOVA and FTIR analysis. Can OriginPro (trial version) perform...
10 July 2023 2,138 0 View
I have the graphs showing DSC-TGA simultaneously but I want to find the glass transition temperature and degree of crystallinity too. Is there some way to calculate or find them from the graphs or...
04 July 2023 3,222 5 View
I want to know whether HDPE is used as labels on plastic bottles of beverages like coke, Pepsi, 7UP, etc.
16 June 2023 1,525 1 View
I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para...
08 June 2023 7,189 0 View
I am performing an esterase assay with cell-free supernatant, and I want to reuse these plates after washing them with distilled water and then, bleach solution. Any suggestion? Can I perform this...
04 June 2023 1,523 0 View
Our Lab's FTIR knob broke. Can it give right results without the knob touching the polymer just by the diamond sensor in FTIR?
20 May 2023 7,520 2 View
I have made a standard curve of p-Nitrophenol concentrations. Now I want to apply the trendline equation on a huge dataset simultaneously to find the unknown concentrations from ODs. Which is the...
15 May 2023 879 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View