In my opinion, the easiest way is to pack the particles in a dialysis membrane of the appropriate cutoff, achieve sink conditions with a buffer containing a surfactant (if necessary) and run a cumulative drug release analysis. Keep the solution in a shaker/waterbath.
The dissolution of nanoparticles into their constituent ions and ion-complexes has been shown to be an important component of the toxicity of some nanomaterials. Silver ions are more toxic than silver nanoparticles on a per mass loading basis and direct correlations of the silver ion concentration of nanoparticle suspensions and toxicity have been made. One model for nanoparticle toxicity is that the nanoparticles provide a high surface area release source for toxic metal ions. Studies have demonstrated that the release rate from nanoparticles is correlated to the surface area per unit mass with larger surface areas (i.e. smaller particles) leading to higher ion release rates.
dialysis could be too slow. You might measure the transit time through the membrane instead of the release. I suggest Syringe filter with a Cut-Off of 0.02µm, there are some models commercially available, e.g. Anotop by GE healthcare.
i think you can put the nanoparticles in the form of powder, capsule or tablet in the vessels of dissolution test of USP apparatus (paddle method prefered). but before analysis of your drug you must filtered the solution or suspension by suitable filter (0.22 micrometer) and measured your dissolution rate.
Watch out: depending on the size of your particles, 0.22µm might be too large. In experiments with nanosized fenofiibrate (Lipidil ONE 145) we saw no difference between 0.02µm and 0.1µm, but content was altered when using 0.22µm, having high SD.
A silver concentration of 40 μg/L was reached after just 6 hours by Tween-coated particles, accounting for ca. 3 % of the total silver. Silver release of Tween-coated particles remained steady until Day -5, when it dropped to a plateau of ca. 30 μg/L. The presence of synthetic sun light and citrate impart significant morphological changes to the particles, however, aggregation seems the controlling process
We have developed a new dissolution test method know as pulsatile microdialysis to characterize nanoparticle and other supersaturated system.This method is 100-1000 time quicker then the general microdialysis (bag). Please follow the attached publication
Article An improved method for the characterization of supersaturati...
another straightforward and fast method for separation is the use of centrifugal filtration. E.g.for nano silver and respective formed ions this method has been verified. Centrifugal filtration tubes are commercially available with various 'cut-offs'. Dialysis might be too slow. Agglomeration or further variations of the nanoparticles during dialysis cannot be completely excluded and therefore may alter the results. If someone is interested and/or have enough time a comparision of both methods (dialysis and centrifugal filtration) is of real great interest. Many authorities and idustries are really interested in that topic.
Another method of choice might be the field-flow-fractionation (Companies; Wyatt, Postnova). However, this method bears a lot of pitfalls and challenges.