i have screen printed electrode and i want to deposit gold nanoparticels on the workig electrode , the particles suspension in citrate buffer. how to do that using electrochemistry?
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screen printed carbon electrode was characterized using 5 mM Ferricyanide/ 5mM Ferrocyanide with 0.1 M KCL , with scan rate from 10 to 100 mV/s vs Ag/AgCl , why the shift go to the right then to...
03 April 2019 8,806 6 View
I have gold nanoparticles suspension in citrate buffer and i want to modify the WE of screen printed electrode with is particles using thiosalicylic acid , if any one faced similar work, please...
03 April 2019 1,779 3 View
i have carbon screen printed electrode and gold nano particles suspension in citrate buffer, how to deposit the particles on the electrode in a way to get uniform distribution?
02 March 2019 8,150 5 View
i have amino acid sequence of protein and I am looking for a free tool can predict the 3d structure, also i have the aptamer of this protein and i want to know how they bind with each other ?
01 February 2019 508 6 View
How should I deposit a mono layer of gold nanoparticles (suspension in citrate buffer ) on a screen printed carbon electrode (purchased) using Langmuir?
31 December 2018 4,666 3 View
hello, iam working on an electrochemical sensor, to detect protein biomarker using DNA prob, i want to design a read out circuit for my sensor, also i want to know what is the differantce between...
10 November 2018 3,954 3 View
Currently Iam working on amperometric biosensor using SPCE (screen printed carbon electrode) but I still confuse to figure out what is the different between this electrode with Interdigitated...
10 November 2018 9,608 2 View
what is the optimum dimensions for interdigitated amperometric Label-free biosensor( Length, width and separation) for detect protein bio-marker ? also what is the best chose for working...
09 October 2018 3,770 2 View
Hello everyone! I am going to start depositing GNPs (diameter around 20nm) on a carbon electrode using Langmuir-Blodgett technique, and I would like to know what is the best solution to start...
09 October 2018 1,139 1 View
hello! iam working on a biosensor design, to detect her2 protein using single strand DNA BIORECEPTOR, i need buffer to unbind thiol-modified oligo from AuNP, also a buffer to unbind PROTEIN-DNA...
09 October 2018 844 2 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View