I'm trying to pack an XK16/70 Column with a Toyopearl hw-50s, but I have some questions.

Question 1. I am not sure how many mL of % sludge should be prepared when packing the column.

ID is 16mm and length height is 70cm. I calculated that it is about π*(1.6cm/2)^2*70cm = 140.7ml, and the manual says that the bed volume of XK16/70 is 105-130 and the bed height is 52.5-65cm. do.

When I checked the Toyopearl manual, they told me to prepare 30-50% slurry as 1.1 x the column volume, so I am going to prepare about 1.1*130ml=143ml of 40% slurry (resin 40% and water 60%). Is it right to prepare like this?

Question 2. I don't know how much pressure and flow rate to give when packing the column. The manual says that 0.5 to 3 bar is recommended, so I am thinking of reducing the pressure to 2 bar based on shimadzu HPLC, but I am not sure how much to set the flow rate. When I asked how to do toyopearl packing on Youtube, it was set to 45ml/min at 2bar, but I'm not sure if I can set it like this too.

Question 3. If there is not enough resin when packing the column, can I fill it up once and open the upper part again to pack more?

Question 4. It would be appreciated if you could provide additional advice on column packing.