How to overcome the low WBCs count while RNA extraction from PB MNCs?

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How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

How to find ECC from radiation pattern on cst ?

i need to find ECC for 4-MIMO antenna using radiation pattern on CST

16 July 2024 7,291 1 View

What are the principles of physical chemistry?

what are the main points

10 July 2024 9,236 5 View

Hello all Can we use different concentrations of lemon or orange extract to grow flowers in ornamental trees and shrubs?

I need more pdf on this topic

02 July 2024 6,988 1 View

Is there a close relationship between the glassbrush bush and its insect pollinator, the honey bee?

I need more information about this topic

27 June 2024 4,057 3 View

How do bees work on glassbrush flowers?

I need pdf information to increase my knowledge of this pollinator, which is honeybees

27 June 2024 3,815 1 View

Welcome What is the scientific name of this climbing plant?

I was confused about the scientific name as well as the local name because of the great similarity between this plant and another climbing plant, especially the morphological shape, which is very...

22 June 2024 9,570 2 View

Can you help me the optimization micro grid using multi-objective?

Optimal sizing microgrid off grid case study ''I choose the case study""so in our research using PV and WT .battery storage and diesel generator .we have the data formation Hourly the solar ir and...

20 June 2024 5,086 0 View

Hello all Do peanut shell residues preserve soil moisture after planting?

I want to confirm the information so I can add it to my simple information

20 June 2024 4,160 0 View

Welcome Find these basic axes in p df form:1.Introduction to Botany:2.Plant Structure and Function?

conservation Conclusion: In summary, "Exploring Botany: An Inclusive Guide to Plant Biology" aims to be an invaluable resource for students, educators, and enthusiasts interested in delving into...

13 June 2024 5,807 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View