Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to slides?

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to glass slides?.

04 January 2021 3,589 2 View

Comparative STUDY: sample of sector A in 5 cities and sector B in whole country for ?

sector A sample is 313, and sector B sample is 200. If I take sector B population in the whole country then sample B's comes = 330 Q. Can I take sector A in 5 cities and sector B in the whole...

27 December 2020 6,930 2 View

Find value of heat transfer coefficient in SI units??

A tube is subjected to air at standard temperature and pressure conditions with velocity 5 m/s. initial temperature of the tube is 980 K and drops to 500 K after interaction with the cool air....

21 December 2020 6,408 3 View

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?. I need a valuable suggestions so Please help me....

30 November 2020 2,469 8 View

No title

23 November 2020 6,277 5 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

How can we define/simulate shell elements (S3/S3R, S4, S4R, SAX1, SAX2) in ABAQUS using a python script while defining bending behavior of composites?

Working with ABAQUS 6.14 for defining the UMAT subroutine for the initial imperfection of shell elements.

19 October 2020 974 1 View

How to approach Emotiv Epoc results and analyze them for results and significance for a study linking education and neuroscience?

Hey all, I am currently a master's student at Queen's University in Canada pursuing in the field of education. My informal thesis idea is to inform education experts through the use of...

02 October 2020 5,030 1 View

How can I couple x-coordinates with y-z coordinates in COMSOL?

Hi, I want to couple x-coordinates with y-z coordinates so that the shape of the whole plate looks same movement. For example, if cross-section of the plate i.e. y-z axis shows deflection then...

25 September 2020 5,941 3 View

Hey, How can I implement Kernal Extreme Learning Machine?

actually I want to develop a system which predicts the accident severity using CNN for feature extraction and Mixture of experts including a variation of ELM Extreme Learning Machine (KELM, CELM,...

18 September 2020 2,011 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View