Can anyone help me to separate my phenolic compound in TLC with any new solvent system ?

My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.

22 January 2021 4,693 7 View

General Researching Question: How to begin research?

I am in the 2nd year of my undergraduate and I am very much interested in doing research in biology, chemistry and physics! I saw research opportunities under my professors but they are too...

24 December 2020 1,475 6 View

G-mmpbsa- file error during one step calculation?

Hello, I am using the one-step approach from https://rashmikumari.github.io/g_mmpbsa/single_protein_ligand_binding_energy.html to calculate binding energy between protein and ligand. However, I...

09 December 2020 7,302 1 View

Which gas is used for Bunsen burner in ASTM D6413 Vertical Flame Test?

I'm trying to make a Vertical Flame Test apparatus for flammability test of PVC based leather. I'm not sure about the type of gas which is to be used for generating the flame using bunsen burner.

09 November 2020 1,808 1 View

How should I design electrodes for electrical stimulation on hydrogel ?

I would like to design custom setup for delivering electrical stimulation to cells cultured on electroconductive hydrogels. I am thinking to use standard tissue culture well plates (12 or 6 well...

26 October 2020 1,099 2 View

Can any one help in finding exact focus area for conducting research on data analysis for smart grid applications?

What can be the area of focus? I request eminent researchers in the field to recommend the algorithms on data analysis for Smart Grid for better understanding. I request to consider open source...

02 September 2020 7,625 8 View

How to decide capsule fill content for dry powder inhalation?

My research is based on DPI however my API is 100mg in dose. how should I decide the Capsule fill content in order to keep 100mg equivalent dose.

19 June 2020 9,862 4 View

Is there any platform where one can complain if a reviewer of a journal makes an insult in his review?

If a reviewer of your article submitted to a journal makes an insult in his review, can the person formally complain about it anywhere?

19 May 2020 1,516 3 View

Can NMP solvent be used for the polymerization of acrylonitrile?

NMP: N-methyl-2-pyrrolidone Usually DMF (Dimethyl Formamide), DMSO (Dimethyl Sulfoxide) and ethylene carbonate have been used for polyacrylonitrile production. Is there any literature on the...

25 April 2020 5,305 15 View

Why Tetrafluoroboric acid diethyl ether complex (HBF4.Et2O) would be used over HCl for protonation in a 9:1 MeOH:H2O mixture?

Hi, I recently came across a paper where the authors protonated their molecule in ACN using HBF4.Et2O. My question is, if I had a solvent mixture of 90:10 MeOH:H2O, would the HBF4.Et2O acid work...

31 March 2020 7,752 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View