Hi,

I am wondering how to normalize my sample value wrt to the house keeping gene in ddPCR. Is it the test sample value (copies per ul) divided by the house keeping gene (copies per ul)?

I found few reports where researcher have used a sum of the test sample value+ house keeping gene to divide the test sample.

Also, do I need to log transform my data or can I keep the scale linear?

Regards

Debashish

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