How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

What are the mechanism of actions of Ag and Cu nanoparticles on the bacteria and fungi?

We are assessing the activity of nanoparticle incorporated fabrics against bacteria and fungi.

24 November 2020 8,686 6 View

How to estimate nitrogen content (%) in plant using pelican equipment's KELPLUS ELITE-EX VA semi automatic kjeldahl distillation unit? ?

I am using KELPLUS ELITE-EX VA semi-automatic Kjeldahl distillation unit for nitrogen estimation. The salesman told me that unlike the older version; this version does not require making up the...

01 October 2020 844 3 View

Why Magnesium Sulfate is used in Loop-Mediated Isothermal Amplification(LAMP) reaction instead of MgCl2?

We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...

22 September 2020 6,916 5 View

Cloning in TRV2 vector: tips?

So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...

26 August 2020 8,364 9 View

Is it possible to hire a portable photosynthesis system in Northern India?

I have an experiment in which I want to estimate the photosynthetic activity and carbon dioxide flux of wheat plant. However, in my institute does not have potable photosynthesis equipment. Is...

11 August 2020 9,701 2 View

Is it possible to publish research paper on plant nutrition having only single year of research work in a Thompson and Reuters IF Journal?

As in Masters of Science in Agronomy in many places have only one year of research work, is it possible to convert it to a paper and publish it in an impact factored journal especially when the...

04 August 2020 4,252 6 View

Has anyone used CABS flex 2.0 for molecular dynamics simulation of docked protein-protein structures?

Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please...

24 April 2020 1,509 1 View

What is the difference between Bubble type and Vapor type Precursor?

I am working with La(PrCp)3 Precursor in my Lab. I am not getting enough thickness after oxide layer deposition with ALD. To solve this issue I want to know about the precursor's chemical...

16 February 2020 5,640 3 View

How to register an indigenous variety in India?

The farmers are cultivating an unregistered land-race for a long period; if I wish to register this variety, what are the legal procedure and how to register it.

14 February 2020 525 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View